The environment-friendly natural dyes recently rejuvenated due to the mutagenic and sensibilizing manner of many synthetic dyes. An attempt has been made to test the inherent bio colorant property and medicinal characteristics of the bark of Castanopsis indica (English name; chestnut and local name; Katus) via green technique using water and ethanol as solvent. Dyeing of fabric was accomplished under optimized conditions with the application of natural (such as Aloe vera, mango's bark extract), and commercial CuSO4 mordant. The extract was tested for antimicrobial properties, phytochemical screening, and characterized by ultraviolet-visible (UV-Vis) and Fourier transform infrared (FTIR) spectroscopy. Most importantly, phenols specifically flavonoids and tannins were found to be present among various phytoconstituents which are responsible to provide colors to the fabric in each dye extract. Besides, two of the extracted dye is demonstrated to be active against fungal strains Candida albicans (C. albicans) and Saccharomyces cerevisiae (S. cerevisiae) to bacterial pathogens. The results revealed the coloring of fabric with water extracted C. indica dye with organic and synthetic mordant is to be bright colored compared to dull color from ethanol extract.