Worldwide, one of the major causes of mortality and morbidity is ischemic strokes. There is considerable memory deficits and cognitive learning among the impairments indicated in the survivors. After ischemia event, the neuroprotective strategies were examined for minimizing these deficits. This study is investigating the protective effect and toxicity of M. oleifera extract on potential oxidative stress in animal model related to ischemia-reperfusion. Also, there are thirty-six male Wistar rats (350–430 g) have been divided into 3 groups, in treatment group, the M. oleifera leaves extracts at 400 mg/kg doses has been orally and daily given 3 times a day in two weeks prior to the occlusion regarding cerebral 2 arteries for 25 mins. Later, blood was drawn from the heart for biochemical analyses regarding potential oxidative stress effects. Ischemia–reperfusion impaired showing M.oleifera extracts supplementation the capability to protect from the oxidative effect; Show the extract have the ability of maintaining the functional levels related to antioxidant enzyme GSH-Px, SOD as well as the vital signs glutathione MDA and GSH.