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176510

Application of the Cloud Point Extraction Method in Spectrophotometric Estimation of Esomeprazole using Diazotised p-Nitroanline and Triton X -114

Article

Last updated: 01 Jan 2025

Subjects

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Tags

Analytical chemistry

Abstract

A simple, sensitive, and precise spectrophotometric method has been developed for the quantification of esomeprazole as pure and in its dosage form (injection). The developed method is based on the diazo-coupling reaction of esomeprazole with a diazotized p-nitroaniline to produce a yellow azo dye soluble in an aqueous medium and gave a maximum absorption at 440 nm. Then using cloud point extraction method by adding Triton X-114 to azo dye solution and heating in water-bath at 50 0C for 25 minutes. The cloudy solution cooled in ice bath, then centrifuged for 20 minutes at 4000 rpm, separation the filtrate by decantation, and finally adding 1ml ethanol. The color of the product changed to pink color (redshift) and the pink-colored solution gave a maximum absorption at 585 nm. The optimization of the method conditions was investigated. Under the optimal conditions, Beer's law obeyed from 1 -20 µg/ml with determination coefficients (R2) equal to 0.9989 and an excellent value of molar absorptivity 1.9929 x 104 with a limit of detection (LOD) of 0.045µg/ml and limit of quantitation(LOQ) of 0.147 μg/ml. The developed method was successfully applied to the assay of esomeprazole in injection dosage with accepted analytical results.

DOI

10.21608/ejchem.2021.76251.3728

Keywords

Cloud point extraction, diazo- coupling, diazotized p-nitroaniline, Esomeprazole

Authors

First Name

Hiba A.

Last Name

Alhfidh

MiddleName

-

Affiliation

Chemistry Department / College of Education of Pure Science/ University of Mosul /Mosul,Iraq

Email

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City

-

Orcid

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First Name

Nabeel S.

Last Name

Othman

MiddleName

-

Affiliation

Chemistry Department /College of Science / University of Mosul / Mosul ,Iraq

Email

nsn20002004@uomosul.edu.iq

City

-

Orcid

0000-0002-5930-3925

Volume

64

Article Issue

11

Related Issue

27064

Issue Date

2021-11-01

Receive Date

2021-05-14

Publish Date

2021-11-01

Page Start

6,243

Page End

6,249

Print ISSN

0449-2285

Online ISSN

2357-0245

Link

https://ejchem.journals.ekb.eg/article_176510.html

Detail API

https://ejchem.journals.ekb.eg/service?article_code=176510

Order

11

Type

Original Article

Type Code

297

Publication Type

Journal

Publication Title

Egyptian Journal of Chemistry

Publication Link

https://ejchem.journals.ekb.eg/

MainTitle

Application of the Cloud Point Extraction Method in Spectrophotometric Estimation of Esomeprazole using Diazotised p-Nitroanline and Triton X -114

Details

Type

Article

Created At

22 Jan 2023