Trimethoprim-MIPs were prepared by using (TMP) as the template as well as allylchloride (AYC) or allylbromide (AYB) as monomer, used (TMPTA) tri methylol propane tri acrylate or ethylene glycol di methyl acrylate (EGDMA) as cross-linker and initiator used (BP) benzyl peroxide. By using different plasticizers (di butyl Phthalate (DBPH), Nitrobenzene (NB), oleic acid (OA) and paraffin) for TMP-MIP1 and (Di butyl sebecate (DBS), Di methyl acrylate (DMA), Tributylphosphate(TBP) and Tris(ethylhexyl phosphate (TEHP) ) for TMP-MIP2. Membranes of MIPs were prepared in PVC matrix. The characterizations of each electrode were determined The Slope range from (55.591 - 40.509) mV/decade, Limit of Detection (1 X 10 -5 - 5 X 10 -6) and Linearity range of electrodes MIPs from (1 X 10 -5 - 1 X 10 -1). Stable Signe of electrode pH from (3-9) and study the selectivity with additives of drugs synthesis (Glucose, Calcium stearate, sodium benzoate and benzoic acid) demonstrate strong selectivity.