At present, the major body of research is focused on weaning the world from fossil fuels. The problem is that the world is running out of fossil fuel. Therefore, an alternative source must be identified. The biofuels are promising alternatives. In the case of petrodiesel, a promising alternative is biodiesel production from algae. The ability of microalgae to generate large quantities of lipids with a fast growth rate made them superior biodiesel producers. Using light-emitting diodes (LEDs) as an energy source in microalgal cultivation was recently increased owing to its large spectrum, endurance, and low-energy utilization. Changes in cultivation conditions, limited capabilities of harvesting light, and self-shading of microalgae were the most important problems. Therefore, the photobiostimulation of algae using LEDs radiation led to an increase in algal growth rate which results in increased lipid production. This research investigated the influence of monochromatic LEDs on the growth of Chlorella sorokiniana microalga. At the first phase, microalgae growth and algal biomass significantly increased under red LEDs [2.3 g/L], blue LEDs [1.8 g/L], green LEDs [0.7 g/L], and white LEDs (0.6) g/L as a control, respectively. At the second phase, microalgal growth and algal biomass significantly increased under red LEDs [2.9 g/L], blue LEDs 2.3 g/L, and white LEDs (1.5) g/L as a control, respectively. The percentage of extracted oil (%) or the yield of extracted oil of microalgae was 10.38 % (white LEDs), 16.94 % (blue LEDs), and 15.55 % (red LEDs) respectively. It was concluded that the photobiostimulation of algae using LEDs led to the enhanced weight of algal biomass, therefore increased of lipids and biodiesel production. The red LEDs were the best one in terms of increasing the weight of algal biomass. The blue LEDs were the best one in terms of increasing the percentage of extracted oil. However, the green LEDs were not effective.