Silver nanoparticles (AgNPs) have wide applications. Production of AgNPs can be occurred through different method chemical, physical, and green methods. The most popular methods are chemical approaches. Marine organisms exhibit a wide range of bioactivity. The present study was designed to establish the biosynthesis of silver nanoparticles from marine crustacean extract of the hard and soft parts of male and female E. massavensis. The microstructure, morphology and optical absorption properties of the nanoparticles were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM) and UV-visible spectroscopy. The formation of silver nanoparticles was confirmed by Uv-Vis absorption and the spectra were observed plasmon bands between 441.79–462.74 nm. XRD results show that the nanoparticles are crystalline in nature. SEM images detected the quasi-spherical AgNPs morphological shape. Silver nanoparticles from marine crustacean extract of the hard part of male E. massavensis (HM4) showed the best results in morphology and particle size. Evaluation of the cytotoxicity of AgNPs (HM4) on different cancer cell lines antiviral, anti-microbial, anti-diabetic, anti-arthritic, anti-aging and anti-inflammatory properties were assessed. AgNPs characterization may be introduced a promising applications in medical aspects.