Two-bath dyeing is currently used to dye cotton/nylon blended fabrics. The cotton/nylon blended fibers were initially pre-treated independently with metal oxides based nanoparticles (NPs) before being dyed with acid and reactive dyestuffs. Two baths were used for dyeing of NPs metal oxides pre-treated cotton/nylon fabric with reactive and acid dyes. The morphological properties of the treated fabrics were explored by scan electron microscope and energy-dispersive X-ray. The impact of pre-treatment with NPs metal oxides on dye-ability, colorfastness, and physico-chemical features has been studied. Colorimetric strength (K/S) and CIE Lab were also explored. K/S values, washing, perspiration, and light fastness of the cotton/nylon sample treated with metal oxide nanoparticles were all higher. Antimicrobial activity was also examined against strains of K. pneumoniae, S. aureus and Candida albicans. The findings indicating that colored fabric had antibacterial potential due to the antibacterial property of NPs metal oxides. Aside from the NPs-treatment colored fabrics with excellent UV protection properties are produced.