Abstract
The current study was designed to investigate HPLC and GC-MS analysis of Saussurea hypoleuca root extract with hepatoprotective and antioxidant activities against paracetamol (PCM) induced liver toxicity in Wistar rats. The root methanolic and ethyl acetate (E.A) extracts (400 mg/kg) were administered orally along with standard and control groups by recommended methods for 14days. Serum biochemical tests were performed after blood sampling at 15th day which has shown that PCM intoxicated liver, drastically elevated the levels of SGOT (serum glutamic oxaloacetic transaminase), SGPT (serum glutamic pyruvic transaminase), ALP (alkaline phosphatase), DB (Direct bilirubin), TB (Total bilirubin), GGT (Gamma glutamyl transpeptidase), TC (Total cholesterol), LDL (low density lipoprotein), VLDL (very low density lipoprotein) and MDA (Malondialdehyde) while reduced the levels of albumin, HDL (High density lipoprotein), TP (Total protein) and GSH (Glutathione) in comparison to normal rats. Co administration of Sausssurea root extracts and silymarin significantly normalize these heptomarkers by reducing the drastically increased levels of SGOT (79.9 IU/mL), SGPT (29.3 IU/mL), ALP (211.9 IU/mL), DB (0.4 mg/dL), TB (0.5 mg/dL), GGT (20.2 IU/mL), TC (135.9 mg/dL), LDL (73.6 mg/dL), VLDL (24.02 mg/dL), MDA (35.2 µg/mL) and increased the levels of albumin (38.2 mg/dL), HDL (7.0 mg/dL), TP (2.7 g/L) and GSH (109.9 µg/mL) which directed that plant root has strong hepatoprotective and antioxidant activities. HPLC and GC-MS profiling indicated the presence of various phytochemicals. All these verdicts entitled that plant root holds resilient hepatoprotective and antioxidant activities.