Eight new mixed ligands metal complexes have been prepared by the reaction of Fe(II), Co(II), Ni(II) and Cu(II) with (1:1:1 molar ratio) of ciprofloxacin - eugenol or levofloxacin - eugenol mixed ligands in alkaline medium. The coordination of the mixed ligands toward metal ions has been anticipated in the light of elemental analysis, molar conductance, spectral infrared (IR), ultraviolet-visible (UV-Vis) and magnetic measurments were bidentate ligands. Electronic and magnetic data suggested the octahedral configuration for all these new complexes. The molar conductance data for the metal complexes show non-electrolytic behavior in DMF solvent and non-electrolytic properties, also they were no conducting electrical current.The biological activity of the prepared complexes was studied with positive and negative Gram-positive bacteria and good antibacterial results.