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156908

Cellulases and animal feed production by solid-state fermentation by Aspergillus fumigatus NRCF-122 mutant

Article

Last updated: 01 Jan 2025

Subjects

-

Tags

Biochemistry

Abstract

A high cellulase-producing fungus was isolated from agricultural waste compost, purified, identified genetically as Aspergillus fumigatus NRCF-122 (accession no. MT318341), and improved by UV-irradiation for cellulase production (named as A. fumigatus mutant-17). Optimal cellulase production conditions were studied using sugar beet pulp “SBP" as the substrate of solid-state fermentation "SSF" technique. The optimal conditions were: initial moisture content ratio (1:2 w/v), incubation temperature (30°C), initial pH of solid culture (10), incubation period (4 days), aeration area (1:100 ratio ((1%)), SBP substrate weight : flask volume of moistened SBP in a 250 mL Erlenmeyer flask, fungal inoculum (seeding) culture age (3 days) and inoculum size (20%, v/w). Studies were also involved the effect of different nitrogen sources where urea is the best nitrogen source for cellulase production. Concerning the application of bioethanol production from yeast, using rice straw cellulose as a source of fermentable reducing sugars), the lingo-cellulosic waste rice straw was first pretreated (with NaOH 1.5%) to reduce crystallinity of cellulose & removing lignin and other inhibitors, enhancing the subsequent process (saccharification) of rice straw cellulose. Then, its cellulose was saccharified enzymatically by cellulases (produced from SBP as substrate (under SSF condition) as substrate under the optimum conditions) resulting in reducing sugars. one of the largest livestock production operating costs is the feed bill. In our study we evaluated the resulted crude protein content of the fermented SBP and the results declared that, the total digest nutrient (TDN) and gross energy performance were increased, where as crude fiber, acid detergent fiber ADF & neutral detergent fiber NDF were decreased, thereby upgrading the feed quality of biologically treated SBP.

DOI

10.21608/ejchem.2021.62744.3347

Keywords

Cellulases, animal feed production, solid-state fermentation, Aspergillus fumigatus NRCF-122

Authors

First Name

Mohamed

Last Name

Fadel

MiddleName

-

Affiliation

Microbial Chemistry Department, Genetic Engineering and Biotechnology Research Division. National Research Centre, Dokki, Giza. Egypt.

Email

mfadel54@yahoo.com

City

Dokki

Orcid

-

First Name

Ahmed A.

Last Name

Hamed

MiddleName

-

Affiliation

Microbial Chemistry Department, Genetic Engineering and Biotechnology Research Division. National Research Centre, Dokki, Giza. Egypt.

Email

ahmedshalbio@gmail.com

City

Dokki

Orcid

0000-0002-9997-9284

First Name

Ahmed M.

Last Name

Abd-Elaziz

MiddleName

-

Affiliation

Molecular biology department, Genetic Engineering and Biotechnology, and Biotechnology Research Division. National Research Centre, Dokki, Giza. Egypt.

Email

ahmedmoselhy2000@yahoo.com

City

Dokki

Orcid

0000-0001-6720-484x

First Name

Manal M.E.

Last Name

Ghanem

MiddleName

-

Affiliation

Molecular biology department, Genetic Engineering and Biotechnology, and Biotechnology Research Division. National Research Centre, Dokki, Giza. Egypt.

Email

d.manalnrc@yahoo.com

City

Dokki

Orcid

-

First Name

Amira M.

Last Name

Roshdy

MiddleName

-

Affiliation

Microbial Chemistry Department, Genetic Engineering and Biotechnology Research Division. National Research Centre, Dokki, Giza. Egypt.

Email

amiraroshdy82@yahoo.com

City

Dokki

Orcid

-

Volume

64

Article Issue

7

Related Issue

25275

Issue Date

2021-07-01

Receive Date

2021-02-12

Publish Date

2021-07-01

Page Start

3,511

Page End

3,520

Print ISSN

0449-2285

Online ISSN

2357-0245

Link

https://ejchem.journals.ekb.eg/article_156908.html

Detail API

https://ejchem.journals.ekb.eg/service?article_code=156908

Order

29

Type

Original Article

Type Code

297

Publication Type

Journal

Publication Title

Egyptian Journal of Chemistry

Publication Link

https://ejchem.journals.ekb.eg/

MainTitle

Cellulases and animal feed production by solid-state fermentation by Aspergillus fumigatus NRCF-122 mutant

Details

Type

Article

Created At

22 Jan 2023