Inorganic sulphate and silicate is required by all mammalian cells to function properly, it is the most abundant anion in the human plasma. Sulphate ions are the major source of sulphur which is considered an important element for sustenance of life as it is present in the essential amino and is required by cells to function properly.This paper aimed to compare the constitutional and physical property of pure hydroxyapatite (HA) doped with different kinds of anions such as sulphate ions (SO42-) and silicate ions (SiO44-). The pure phase of HA, SHA (SO42- doped HA), and SiHA (SiO44- doped HA) were successfully synthesised using microwave-assisted precipitation technique method. The acquired materials were recognize using X-ray diffractometer (XRD), Fourier transforms infrared spectroscopy (FTIR), Field emission scanning electron microscopy (FESEM), and energy-dispersive X-ray (EDX) techniques. The consequence shows that incorporation of SO42- and SiO44- ions revealed shrinkage along with a-axis and expansion with c-axis. FTIR show diminished in the intensity of the OH group with an addition of anions. The particles shape significantly decreased with incorporation of SiO44- ions and changed to rod shape with addition of SO42-. The Ca/P ratios were in acceptable values. Based on the outcomes, demonstrated that the anions were successfully integrated in the HA crystal with no decomposition in HA phase.