Screen printed electrode (SPE) is considering one of the most prospective techniques for sensing purposes due to its low cost, easy fabrication and reusability. It is worthwhile to use SPE as a sensor for fast detection of the biogenic H2S gas which is a sign for sulfate-reducing bacteria (SRB) existence. SRB presence is the first indicator in the industrial sector that causes a catastrophic environmental issue which give time for the decision makers to avoid their consequences. Polyaniline (PANI), PANI/MOx nanocomposites were prepared as sensing materials. The prepared materials have been characterized by FT-IR, Raman spectroscopy and GPC techniques. The morphological properties have been determined using SEM and HR-TEM. The particle size distributions have been determined using dynamic light scattering (DLS). Moreover, the fabricated sensors also have been applied as biogenic H2S gas detector using the electrical resistance changes. The data confirmed that the PANI doped with ZnO NPs has the best sensing performance compared to the other fabricated sensors.