In this work new substituted of 2-amino-3-cyanopyridine derivatives were synthesized by reaction between chalcone derivatives and malanonitrile in the presence of ammonium acetate to give excellent yields and new complexes with these ligands were synthesized and characterized by elemental analysis, UV, IR, Mass and NMR (1H, 13C) spectroscopy and had general formula: [ML2(H2O)2]Cl2 where M= Co(ІІ), Ni(ІІ) and Cu(ІІ), [ML2(H2O)Cl]Cl2 where M= Cr (ІІІ). All of these studies indicate that ligand coordinated with ions metals as a bi-dentate ligand and also suggested that prepared complexes had an octahedral geometry coordinated via the carbon (cyano group) and nitrogen (amino group).The antimicrobial assessment of the ligand and its complexes was performed against gram negative such as (Pseudonomous aerugionosa) and gram positive such as (Bacillus subtitis) grams. All these complexes are indicated to show antibacterial effective better than their respective ligands