During the last decade, nanoparticles could be considered as important platforms to treat skin wounds. Metal nanoparticles especially silver have shown potential therapeutic effects on wound healing. In this work, solid state synthesis was used for the preparation of AgNPs without using an extra chemicals or solvents. AgNPs was easily synthesized using dextran as a reducant and stabilizing agent by eco-grinding artlessly for dextran powder, sodium hydroxide beads and silver nitrate with no considerable times. The efficiency of dextran as dual role for the synthesis of AgNPs was evaluated via changing the utilized concentration of silver nitrate. The formed silver nanoparticles were characterized by ultra-violet spectroscopy, transmission electron microscopy, dynamic light scattering, zeta potential, X-Ray Diffraction, field emission scanning-electron-energy dispersive X-ray tools. The resultant AgNPs with different concentration were applied to cotton fabrics. The cytotoxicity and antimicrobial activities of the treated fabrics was evaluated. The results revealed that the spherical shape with well distribution of prepared AgNPs was affirmed. In addition, the stability of AgNPs was achieved and acquired values more than -30 mv. The results also depicted that the nanoparticles were successfully deposited onto and penetrated inside the surface of fabrics. The treated cotton fabrics exhibit superior antimicrobial properties that determined by disc diffusion method to kill the pathogenic microbes. Additionally, the cell viability (%) of the treated cotton fabrics has no toxicity effect for the human and non-human cell lines. Based on that, the synthesis of AgNPs is based on solvent-free and one pot synthesis of silver nanoparticles which is eco-friendly, low cost, save effort and chemicals, also gives a high yield.