Bladder cancer was considered the third most common tumor in Egypt according to the National Cancer Institute (NCI) registry. However, Transitional cell carcinoma of the urinary bladder represents about 90% of all bladder cancer cases. Method; The aim of the study was to evaluate the expression levels (mRNA and protein) of E-cadherin and special AT-rich sequence binding protein-1 (SATB1) as biomarkers for transitional cell carcinoma (TCC) using immunohistochemistry and quantitative reverse transcription PCR (RT-qPCR) techniques of the Egyptian urinary bladder patients compared to the noncancerous bladder tissues. The study included 48 TCC tissues (41 males and 7 females) of different grades and stages and 12 noncancerous bladder tissues (7 males and 5 females). Results; A statistically significant association regarding smoking and gender were found between the bladder transitional cell carcinoma (BTCC) group and the control group. However, there was no statistically significant difference in comparing the presence of schistosomiasis in both studied groups. The expression of E-cadherin was significantly down expressed and SATB1 was significantly over expressed in TCC tissues compared to the noncancerous bladder tissues at both mRNA and protein levels. Decreased expression of E-cadherin and over expression of SATB1 were statistically associated with tumor stage, tumor grade, and tumor size. On the other hand, no statistically significant difference was detected between the expressions of the two studied genes and either gender or age. A significant association was found between the protein expressions of E-cadherin and SATB1. Pearson correlation test showed significantly negative correlation between mRNA expressions of E-cadherin and SATB1. Conclusion; E-cadherin and SATB1 expressions may be considered as good biochemical markers for the diagnosis and prognosis of transitional cell carcinoma in urinary bladder of Egyptian patients.