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130405

Chemical and Biological Activities of Deverra triradiata Hochst. ex. Boiss. Aerial parts from St.Catherine, Southern Sinai, Egypt.

Article

Last updated: 22 Jan 2023

Subjects

-

Tags

Organic chemistry

Abstract

The present study describe the phenolic and flavonoid profiles of the ethyl acetate extract of D. triradiata which have been demonstrated by high-performance liquid chromatography (HPLC) to prove the presence of 15 compounds, nine major compounds namely, querectin (46.37 mg/g), vanillin (11.94),daidzein (10.02),ellagic acid (4.76), propyl gallate (3.89), naringenin (3.27), gallic Acid (3.18 mg/g), rutin (2.7) and chlorogenic Acid (2.58). The chemical constituents of unsaponifiable portion of petroleum ether 60-80o extract which analyzed by gas-liquid chromatography (GLC) proved the presence of 14 hydrocarbons and three sterol; n-eicosane (16.94%), n-docosane (8.22 %), n-heptadecane (7.13 %),squalene (6.89%), n-tricosane (1.63 %), n-hendecane (1.63 %), cholesterol (1.12 %), campesterol (3.81 %) and stigmasterol (5.27 %).The saponifiable portion five unsaturated fatty acids were indicated as,α-Linolenic acid (17.95 %), arachidonic acid (11.37), linolenic acid (6.12),oleic acid (3.36) and palmitoleic acid (3.01) and also four saturated fatty acid named by, palmitic acid (31.61), myristic acid (7.17), Lauric acid (1.67) and stearic acid (1.47).Total phenolic (tannic acid equivalent), total tannins (tannic acid equivalent) and total flavonoids (quercetin equivalent) were (67.8±0.39, 31.4 ± 0.63 and 4.7 ± 0.077 mg/g d.w.), respectively. The in vitro antioxidant activity using the stable free radical DPPH (2,2-diphenyl-1-pycrylhydrazyl) method of the petroleum ether and ethyl acetate extracts . The percentage of maximal inhibition for extracts in DPPH are 18.42 ± 0.39 and 43.58±0.52 %, respectively compared with percentage of gallic acid 91.29 ±0.72μg/ml, which proved that ethyl acetate extract possesses a distinct radical scavenging effect, which may be attributed to its high polyphenolic content.The in vitro cytotoxic activity by using SRB assay, of petroleum ether showed promising activity against (MCF7), (PC3) and (HCT 116) with IC50 18.8, 9.3 and 9.5 ug/ml, respectively, compared with normal cell. Baby Hamster kidney (BHK) with IC50 37, while, the ethyl acetate extract showed weak activity against (MCF7), (PC3) and (HCT) with IC50 42, 42 and 37 ug/ml, comparable to (BHK) normal cell with IC50 59 μg/ml. All identified phenolic compounds are therapeutically active compounds with anticancer potency and antioxidant capacities which were reported for the first time.

DOI

10.21608/ejchem.2020.52846.3092

Keywords

Deverra triradiata, HPLC, GC, Total phenolic, Flavonoids, antioxidant, Cytotoxicity

Authors

First Name

Amani

Last Name

Elmosallamy

MiddleName

-

Affiliation

Zagazig university department of Organic chemistry

Email

amanimd@hotmail.com

City

-

Orcid

0000-0003-2853-6870

First Name

Enas

Last Name

Ibraheem

MiddleName

-

Affiliation

Deparment of Medicinal and Aromatic plants, Desert Research Center, Mataryia, Cairo, Egypt

Email

enasibraheem59@yahoo.com

City

-

Orcid

-

First Name

Mohamed

Last Name

Sarhan

MiddleName

-

Affiliation

Medicinal and Aromatic Plants Department, Desert Research Center, Cairo, Egypt

Email

srhanfacebook@yahoo.com

City

-

Orcid

-

First Name

Sahar

Last Name

Hussein

MiddleName

Awad Allah

Affiliation

Head of department of Phytochemistry and Plant Systematics,Division of Pharmaceutical Industries,National Research Center, El Buhouth st., Dokki Dokki, Cairo, Egypt

Email

drsahar90@yahoo.com

City

Cairo

Orcid

0000-0002-6305-508X

Volume

64

Article Issue

3

Related Issue

21851

Issue Date

2021-03-01

Receive Date

2020-12-09

Publish Date

2021-03-01

Page Start

1,387

Page End

1,394

Print ISSN

0449-2285

Online ISSN

2357-0245

Link

https://ejchem.journals.ekb.eg/article_130405.html

Detail API

https://ejchem.journals.ekb.eg/service?article_code=130405

Order

28

Type

Original Article

Type Code

297

Publication Type

Journal

Publication Title

Egyptian Journal of Chemistry

Publication Link

https://ejchem.journals.ekb.eg/

MainTitle

-

Details

Type

Article

Created At

22 Jan 2023