The application of biotechnology in the textile processing is gaining worldwide detection as one of the hopeful approaches to pollution issue and cost reduction . Hydrolase enzymes as amylases , pectinase and cellulase have been successfully used in the wet process over the past 40 years. The application of these enzymes in one bath is the new challenge. laccases enzymes are used in biosynthesis and decolourization of textile effluents. Application of enzyme in hydrolysis of starch thickeners which is the main component of printing paste enzyme are one of the promising area. Green synthesis of dyes, in-situ coloration, polymer grafting are the new trend of enzymes application as it produce smart colored textile. Application of biotechnology in textile is the solution of almost all the problems of this industry. The enzymes researches illustrate improvement in product quality at shorter times and lower energy and water consumption. This state of art highlights the novel approaches of application of enzymes in the textile coloration and industry covering both current researches and pilot application.