Sensitive disposable screen printed potentiometric vardenafil (VAR) sensors have been constructed based on multiwall carbon nanotubes/crown ether (MWCNTs/CE) composite. Factors affecting the sensor performance have been optimized on the basis of nature and content of the molecular recognition elements, anionic sites, membrane plasticizers and nanomaterial. The fabricated sensors showed acceptable selectivity and sensitivity within the vardenafil concentration range from 10-6 to 10-2 mol L-1 with Nernstian slope value of 62.1±0.8 mV decade-1 and detection limit 8.0 × 10-7 mol L-1. Fast response time, enhanced potential reading stability and long shelf lifetime are the promising futures of the novel sensors. The proposed method has been suggested for potentiometric assay of VAR in dosage formulation and biological fluids with average recoveries agreeable with the reported official methods.