The reactions between Tretinoin (Tret) and Isotretinoin (Itret) isomers with Iodine were thoroughly investigated under different conditions and solid products under proper conditions were prepared. Separation and identification of the formed products approved the stoichiometry (1:1). These newly prepared solid products were separated, washed, crystallized and structurally investigated and identified. Structures of the newly synthesized retinoid-iodine derivative products were carefully investigated in this research. Their structures were characterized, elucidated and analyzed by using the mass spectrometry (MS), Fourier Transform Infra-Red (FT-IR), 1H-NMR, thermal analyses (TA) and X- Ray Fluorescence (XRF). Then the results of the newly prepared compounds were compared with that of parent isomers analyses. Therefore; the proposed general formulae of the newly prepared compounds were found to be C20H27IO2. The biological activities of the newly obtained products in comparison with the parent isomers are thoroughly investigated against different kinds of bacteria and fungi.