Phenolic compounds are classified as hazardous materials that polluted groundwater and surface water resources. The present work have been applied photo- Fenton process for removal of phenolic wastewater, also it concerned with the optimization of the operating parameters that affect the photo-Fenton process. Using the Response Surface Methodology (RSM) to explore the influence of operational variables such as hydrogen peroxide and the iron ion weight ratio, pH, contact time on the removal of phenol. The predicted removal obtained from the model are in a good agreement within the results of experiments with a high correlation regression of 0.993. The expected maximum removal was to be 80.12 % while the experimental results was 81%. The study estimated the influence of [Fe2+/H2O2] weigh ratio from 20-60, contact time from 30-90 min and pH range from 2 to 8, at solution containing of 100ppm phenol. A Box–Behnken design was applied to optimize the operating parameters. The graphical response surface and contour plots were employed to determine the optimum conditions. The results showed that the optimized value of the [H2O2/ Fe2+] = 40.4, contact time= 55 min, and pH=5.2 giving a reduction efficiency of 80.12%.