This study aimed to evaluate the hypolipidemic, and anti-obesity effects of polyunsaturated oils, mixed with gold nanoparticles (AuNPs). AuNPs were prepared by the oleic acid pyrolysis method. The sample was characterized by UV spectroscopy and transmission electron microscope (TEM). Sixty adult male rats were divided into 2 main groups. Group one (n=10 rats) was fed on the healthy diet (serves as basic control). The other group was fed on the high fructose diet (HFD) for four weeks and then was divided into five subgroups. 1st subgroup was fed on HFD only, 2nd and 4th subgroups were treated with n-3 PUFAs oils while 3rd and 5th treated with n-3 PUFAs oils mixed with AuNPs. Treatment of obese rats with n-3 PUFAs mixing with AuNPs revealed a significant decrease in in weight gain accompanied by an improvement of lipid profile. Histological examination of the heart indicated a marked improvement in the architecture and marked decrease of relative weight of heart in treated rats. It may be concluded that mixing of PUFAs oils with AuNPs could have the ability to lower weight gain of obese rats and relieved the various biochemical and histological abnormalities resulted due to obesity metabolic disorders.