In this study, a library of Schiff bases derived from aliphatic (Glyoxal) and aromatic (Vanillin) aldehydes with different substituted aniline compounds. In addition to their complexes with Iron (III) were synthesized. The synthesized compounds were characterized by chemical and physical techniques (IR, 1H-NMR, melting point, color, UV-Visible). The library of the Schiff bases and their complexes were tested against two types of human pathogen bacteria (Staphylococcus aureus (Gram-positive) and Escherichia coli (Gram-negative)). in a public way, The Fe+3 complexes were relatively more active against Escherichia coli than the corresponding Schiff bases. The antibacterial activities of the Schiff bases and their complexes against Staphylococcus aureus were variable.