This work focuses on the preparation of immobilized ivermectin (IVM) and essential oil carvacrol (Cav) as ‎models of antiparasitic drugs on functionalized multi-walled carbon nanotubes (MWCNTs) using sol-gel technique. ‎Fourier transform infrared spectroscopy (FTIR), transmission electron microscope (TEM) and particle size ‎distribution analysis were used to characterize the prepared materials. In addition, the in vitro cytotoxic effect was ‎investigated against normal fibroblast cell line (BHK-21) using SRB assay. Also, toxicity to Rhipicephalus ‎annulatus female ticks was also performed in vitro. The FTIR and TEM results showed that the drugs loaded on ‎the functionalized MWCNTs were successfully prepared through covalent bonding with a particle size range of ‎around 407 and 268 nm in the case of IVM and Cav, respectively. The cytotoxic activity showed that the drugs ‎loaded had low cytotoxic effects of about 4.5 and 4.4 % relative to the free IVM (8.5 %) and Cav (7.8 %), ‎respectively, at 100 μg/mL concentration. In addition, the loaded drugs displayed high ticks mortality at about 100 ‎‎% compared to the free IVM (23.3 %) and Cav (26.7 %), respectively at 250 μg/mL concentration after 72 h of ‎exposure. ‎