The purpose of the present study is to biosynthesis of ethanol extracts from The Ripe fruits of Melia azedarach plant to investigate the ecofriendly and economic efficient and apply this extract on cotton fabrics and its blend to impart antimicrobial efficacy by printing style under different concentration . Where the fabric samples were pre-treated with tannic acid which used as amordant . The Results of GC-MS analysis showed that the ethanol extract of M. azedarach contains 27 compounds and it is rich in many substances such as tannins, flavonoids and other phenolics compound , also results showed that the crude extract of Melia azedarach possess antimicrobial activity against Gram negative and Gram positive bacteria . The results of scanning electron microscope ( SEM) indicated that there is a difference in the surface shape of the treated and untreated fabrics. As well the treated fabrics showed good inhibitory activity against Gram positive bacteria with inhibition zones range from 7 to 12 mm and Gram negative one with inhibition zones range from 7 to 15 mm.