The aim of this paper was to research how coating of Egyptian cotton fabric with renewable source of natural amino acids extracted from soybean seed waste would influence the textile to be coated and how it affects the penetration of reactive dyestuff. Cationization have been studied on scoured and mercerized cotton fabrics for achieving electropositive charge and better adsorption properties and the optimum conditions were determined. The changes in surface morphology of fabrics after cationization were identified by FTIR spectroscopy analysis. The results revealed obtained that the color measurements, tensile strength, elongation and fastness properties of cationized cotton fabrics has improved compared with the untreated fabrics. An important advantage of cationic treatment is in the economical and environmental impact by using the waste of soybean and minimize the environmental problems occurred in reactive dyeing by making salt-free reactive dyeing.