An effective novel electrochemical sensor constructed for the determination of Midodrine hydrochloride drug using differential pulse voltammetry. The sensor is based on a carbon paste electrode modified with gold nanoparticles and β-cyclodextrin in Britton-Robinson buffer of pH 3.0. The effect of various experimental parameters on the constructed electrodes properties including pH, β-cyclodextrin concentration and scan rate was investigated. At the optimum conditions, a linear range from 3.0 x 10-6 to 3.2 x 10-4 mol L-1 with correlation coefficient of 0.9997 and a detection limit of 5.14 x 10-7 mol L-1were obtained. The modified electrodes were used for microdetermination of the drug in tablets and spiked human urine samples with appropriate an acceptable recovery, reproducibility, selectivity and robustness. Potential strength investigation was achieved using 0.1 M of HCl.