Today, natural colorants are emerging globally due to the fact that they are safer and more environmentally friendly. In this study, the dyeing substance produced by Streptomyces Thinghirensis strain (EGDA6S) was extracted by using the solvent extraction method. The extracted dyestuff was used for one step dyeing and antimicrobial finishing of wool fabrics. The dyeing properties of the dyestuff under study were investigated and the color uptake expressed as color strength was evaluated. The results revealed an excellent color uptake as well as excellent color fastness (washing, rubbing, light) that ranged (4-5). Also, the antimicrobial test showed that dyed samples have an excellent potentiality against tested pathogens.