Gelatin-tannic acid (bio-mordant) treated cotton fabric was printed with natural and synthetic thickeners. Madder natural dye was used with different thickeners include meypro gum, carboxymethyl cellulose, sodium alginate, or acrylate-based synthetic thickener. Different factors such as pH, steaming temperatures, time, auxiliaries (urea, salt, and binder) presence or absence in the print paste formulations were investigated. The data indicated good quality prints using natural thickeners and without auxiliaries compared with the synthetic one. The printed samples reveal very good to excellent fastness properties. Hence, the present process of printing cotton fabric with green print paste formulation and without auxiliaries may find wide application in textile coloration.