A SCHIFF base 3 was synthesized and used as a new capping agent for synthesis of silver nanoparticles through preventing overgrowth of silver oxide. Schiff base 3 helps to enhance the structural properties of the resulted silver nanoparticles in a distinguishable style. The structure of Schiff base 3 was confirmed on the basis of spectral data (1H nuclear magnetic resonance, mass, and Fourier-transform infrared spectroscopy) and melting point. The ultraviolet–visible was used to monitor the formation of silver nanoparticles within a surface plasmon band at 400 nm. Scanning electron microscopy image shows the particle size of silver nanoparticles which have a spherical shape (6-45 nm). The resulted silver nanoparticles were screened against four types of bacteria: Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae and Escherichia coli.