Exploring novel and effective therapies against liver damage with antioxidant activity is a point worth studying. The present study was aimed to evaluate the hepatoprotective and antioxidant activities of Euphorbia cooperi N. E. Br. aerial parts in paracetamol-induced hepatotoxicity mice and isolates its bioactive constituents. Our findings suggest for the potential antioxidant and hepatoprotective action of the n-butanol fraction of the plant as significantly (p < 0.05) prevented the increased serum marker enzymes AST, ALT and bilirubin levels, decreased hepatic MDA and recovered GSH levels. Chromatographic separation of n-butanol fraction led to isolation of seven compounds namely, Gallic acid, Bervifolin carboxylic acid, Kaempferol-3-O-ß-D-rutinoside, Corilagin, 3, 3'-dimethoxy ellagic acid, 3, 4, 4'-trimethoxy ellagic acid and Ellagic acid.The structure of isolated compounds was elucidated by physical and spectroscopic analysis and by comparison with the literature data. These compounds isolated for the first time from Euphorbia cooperi N. E. Br.