Printed cotton fabrics using different concentration of honey gum( propolis )inside printing paste Propolis is a gum has been used as a traditional cure for various diseases due to a variety of biological activities of this folk medicine. In the present work, an attempt made to investigate antibacterial formulation. The interaction between printing paste containing propolis and cotton was confirmed using FTIR. Morphological properties and printing pigment distribution was examined using SEM. Printing color strength and fastness properties were examined. Antibacterial activity was explored against gram positive, negative and yeast. ATR/IR showed different intensities at OH region that confirm interaction between propolis and fabrics and in comparison with fabric and printed cotton without propolis. Thick coating and highly distributed fabrics was visualized at SEM images at printed cotton fabrics containing propolis. The color strength of printed cotton fabrics containing propolis was directly proportional with propolis content up to 4%. Printed cotton fabrics containing propolis showed stability at all fastness evaluations. Printed cotton fabrics containing 4% propolis showed efficient antimicrobial activity towards both gram positive and negative bacteria