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31532

Enhancement of Diazinon Degradation, Isolation of Organophosphorus Hydrolase and Chemical Analysis of Metabolites

Article

Last updated: 01 Jan 2025

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-

Tags

Environmental chemistry

Abstract

In this study, diazinon high degradation was achieved by using Ethyl methane sulfonate (EMS) as a chemical mutagen, 38.19% of diazinon was degradedin the minimal media as compared to 1.83% in control media after 5 days.Due to the first step of ethylmethane sulphonate (EMS) chemical treatment, diazinon degradation increased to 62.19% which was subsequently increased to 86.21% after the EMS treatment second step. Analysis of organophosphorus hydrolaseprotein involved in diazinon hydrolysis revealed that itis a member of the MBL-fold metallo hydrolase superfamily and has beta lactamase fold.2-Iso-4-methyl-6- hydroxypyrimidine (IMHP) was detected as the main degradation product of diazinon through GC/MS analysis after 5days of incubation and further metabolized to unknown polar metabolites that weredetected using GC/MS after 10 days.

DOI

10.21608/ejchem.2019.11689.1748

Keywords

OPs pesticides biodegradation, Diazinon, EMS, GC/MS analysis

Authors

First Name

Nivien. A.

Last Name

Abosereih

MiddleName

-

Affiliation

Department of Microbial Genetics, National Research Centre, 33 El-Bohouth St., Dokki, Giza 12622, Egypt.

Email

nivienabdelrahman@yahoo.com

City

-

Orcid

0000-0002-2513-3294

First Name

Ghada. M.

Last Name

El-sayed

MiddleName

-

Affiliation

Department of Microbial Genetics, National Research Centre, 33 El-Bohouth St., Dokki, Giza 12622, Egypt.

Email

-

City

-

Orcid

-

First Name

S. A.

Last Name

Ibrahim

MiddleName

-

Affiliation

Department of Genetic, Faculty of Agriculture, Ain Shams University, Cairo, Egypt.

Email

-

City

-

Orcid

-

First Name

A. B.

Last Name

Abd El-Razik

MiddleName

-

Affiliation

Department of Genetic, Faculty of Agriculture, Ain Shams University, Cairo, Egypt.

Email

-

City

-

Orcid

-

First Name

M.A.

Last Name

Hammad

MiddleName

-

Affiliation

Department Plant Protections, Faculty of Agriculture, Ain Shams University, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

F. M.

Last Name

Hafez

MiddleName

-

Affiliation

Department of Microbial Genetics, National Research Centre, 33 El-Bohouth St., Dokki, Giza 12622, Egypt.

Email

-

City

-

Orcid

-

Volume

62

Article Issue

11

Related Issue

8549

Issue Date

2019-11-01

Receive Date

2019-04-10

Publish Date

2019-11-01

Page Start

2,131

Page End

2,142

Print ISSN

0449-2285

Online ISSN

2357-0245

Article File

EJCHEM_Volume 62_Issue 11_Pages 2131-2142.pdf

PDF . 1.8MB

Link

https://ejchem.journals.ekb.eg/article_31532.html

Detail API

https://ejchem.journals.ekb.eg/service?article_code=31532

Order

13

Type

Original Article

Type Code

297

Publication Type

Journal

Publication Title

Egyptian Journal of Chemistry

Publication Link

https://ejchem.journals.ekb.eg/

MainTitle

Enhancement of Diazinon Degradation, Isolation of Organophosphorus Hydrolase and Chemical Analysis of Metabolites

Details

Type

Article

Created At

22 Jan 2023