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28326

Microwave Assisted Peptide Synthesis of Some Rationally Designed Cell Penetrating Peptides from C-Kit Receptor

Article

Last updated: 01 Jan 2025

Subjects

-

Tags

Organic chemistry

Abstract

In the interest of public health, cell-penetrating peptides (CPPs) have proven to be important way for the highly efficient intracellular delivery of bioactive cargos like peptides, proteins, and oligonucliotides. It is feasible to combine one or multiple CPPs with bioactive cargos either by direct chemical conjugation or, more rarely, as non-covalent complexes. The term bioportides was introduced to describe monomeric CPPs that are intrinsically bioactive. One of the consistent problems that may compromise the in vivo applications of CPPs and bioportides, is the stimulation of mast cell (MC) secretion. Therefore, in this study two decapeptides, S6 and S6P, has been designed from the c-kit protein, a mast cells receptor, and synthesized using the technology of microwave assisted solid phase peptide synthesis. In an attempt to find bioportides from the c-Kit protein, and studying their structure- activity relationship, the present work was carried out, investigating the effect of the presence of the phosphotyrosine moiety as an active site in the synthesized peptides.

DOI

10.21608/ejchem.2019.7119.1611

Keywords

Microwave assisted solid phase peptide synthesis, Cell-penetrating peptide, Bioportides, C-Kit receptor

Authors

First Name

Shaimaa

Last Name

Osman

MiddleName

-

Affiliation

Peptide Chemistry Department, Chemical Industries Research Division, National Research Centre, Cairo, Egypt.

Email

shaima.mahdy@gmail.com

City

-

Orcid

-

First Name

Sarah

Last Name

Jones

MiddleName

-

Affiliation

Research Institute in Healthcare Science, School of Applied Sciences, University of Wolverhampton, Wolverhampton, UK

Email

s.jones80@wlv.ac.uk

City

wolverhampton

Orcid

-

First Name

Mohamed

Last Name

Zewail

MiddleName

-

Affiliation

Peptide Chemistry Department, Chemical Industries Division, National Research Centre, Cairo, Egypt

Email

zewail40@gmail.com

City

-

Orcid

-

First Name

Abdelgawad

Last Name

Rabie

MiddleName

-

Affiliation

Chemistry Department, Faculty of science, Ain Shams University, Cairo, Egypt

Email

mohamedabdelgawad@hotmail.com

City

-

Orcid

-

First Name

Ahmed

Last Name

Shalaby

MiddleName

-

Affiliation

Peptide Chemistry Department, Chemical Industries Research Division, National Research Centre, Cairo, Egypt

Email

ahshalaby45@gmail.com

City

-

Orcid

-

First Name

John

Last Name

Howl

MiddleName

-

Affiliation

Research Institute in Healthcare Science, School of Applied Sciences, University of Wolverhampton, Wolverhampton, UK.

Email

jh.howl@wlv.ac.uk

City

wolverhampton

Orcid

-

Volume

62

Article Issue

8

Related Issue

6668

Issue Date

2019-08-01

Receive Date

2019-01-27

Publish Date

2019-08-01

Page Start

1,377

Page End

1,389

Print ISSN

0449-2285

Online ISSN

2357-0245

Link

https://ejchem.journals.ekb.eg/article_28326.html

Detail API

https://ejchem.journals.ekb.eg/service?article_code=28326

Order

3

Type

Original Article

Type Code

297

Publication Type

Journal

Publication Title

Egyptian Journal of Chemistry

Publication Link

https://ejchem.journals.ekb.eg/

MainTitle

Microwave Assisted Peptide Synthesis of Some Rationally Designed Cell Penetrating Peptides from C-Kit Receptor

Details

Type

Article

Created At

22 Jan 2023