In present work, different natural fabrics (cotton, wool and silk) were printed via pomegranate peel dye. The dye solution subjected to ultrasonic to reach the nano size. Two techniques of mordanting were used, (pre-mordanting and simultaneous mordanting); substrates were mordanted prior to printing process using two ecofriendly mordants separately: Alum and tannic acid, other substrates were printed via paste incorporated with mordant for comparison. Pomegranate peel natural dye was studied to clarify the impact of nature of nano-size color particles on size, shape, and particle distribution of the natural dye with comparative studies of the K/S and over all fastness properties of printed samples. Results showed that the K/S values of nano samples are higher than original samples, irrespective of the nature of the fabric used and /or the concentration of the coloring matter. Nano-pomegranate peel dye could be used successfully instead of the original dye incorporated with Alum mordant irrespective
of the fabric used. In addition, pre-mordanting technique acquired the higher K/S values than the simultaneous mordanting technique. There is a tendency of improvement of the perspiration fastness while keeping the washing and rubbing fastness unaltered after miniaturization of the pomegranate dye.