This study deals with the preparation and characterization of Nanofiltration cellulose acetate-based membranes and their application in desalination of brackish water. Cellulose acetate nanofiltration (CA-NF) membranes were prepared using phase inversion process. Pore size of the prepared membrane was checked via dope solutions of different polymer concentrations (wt. %) and treatment temperatures from 60-90°C. followed by casting the membrane on Polytetraflouroethylene sheets (PTFE) and examining the efficiency of the product. Characterization of the prepared membrane was carried out utilizing scanning electron microscopy (SEM). The work was extended to study the hydraulic permeability, water permeation as well as salt rejection percentage. Characterization of the prepared membrane will be carried out utilizing scanning electron microscopy (SEM), the hydraulic permeability, water permeation as well as salt rejection percentage. Results indicated that CA membranes reinforced with (PTFE) gave better efficiency than normal CA membranes.