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9151

Enhancement of Crude Oil Biodegradation by Immobilized Bacterial Consortium in Small Batch and Continuous Bioreactor Modes

Article

Last updated: 22 Jan 2023

Subjects

-

Tags

Environmental chemistry

Abstract

Oil spills besides the discarding of oil refineries byproducts directly into the environment is a major source of nature pollution which contributes to environmental contamination and health problems. Firstly for solving this problem oil degrading bacterial consortium (Pseudomonas sp. sp48 and Bacillus cereus M12 which were isolated from soil samples from Bahary area, SidiKerir branch, Alexandria, Egypt, respectively) was immobilized with different carriers, where rice straw was selected as the best one for oil degradation. Conditional optimization for immobilized bacterial consortium has been explored by testing various amounts of preculture (2, 3, 4, 5.5 and 7%), different incubation time with rice straw (1-5 h), enumeration of viable cells immobilized and different oil concentrations (1, 2, 3, and 4%). The degradation of crude oil (1% concentration) reached to 61.6% due to biological action alone while it raised to 97.14% by binary actions of biological and physical after 6 days incubation time and applying the optimum conditions (4% bacterial consortium preculture and 3 h incubation time with the carrier). Finally application study has been carried out using the ideal model conditions in small batch bioreactor and continuous to treat oil contaminated water in an open system. The results showed that the crude oil degradation biologically only and biologically/ physically reached to 56.2% and 87.2%, respectively. Whereas the continuous addition of 1% oil concentration for three times the removal reached to 81.27%. This technique of immobilization produces a promising result for solving the oil pollution problem due to the collaborating effects for both physical and biological actions.

DOI

10.21608/ejchem.2018.3820.1336

Keywords

biodegradation, crude oil, Bacteria, Immobilization, Bioreactor

Authors

First Name

Soha

Last Name

Farag

MiddleName

-

Affiliation

Environmental Biotechnology Department, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological Applications, Alexandria, Egypt

Email

sohafarag88@yahoo.com

City

Alexandria

Orcid

-

First Name

Nadia

Last Name

Soliman

MiddleName

A

Affiliation

Bioprocess Development Department. Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological Applications, Alexandria, Egypt

Email

nadiastuttgart@yahoo.com

City

-

Orcid

-

First Name

Yasser

Last Name

Abdel-Fattah

MiddleName

R

Affiliation

Bioprocess Development Department. Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological Applications, Alexandria, Egypt

Email

yasser1967@yahoo.com

City

-

Orcid

-

Volume

61

Article Issue

6

Related Issue

2055

Issue Date

2018-12-01

Receive Date

2018-05-16

Publish Date

2018-12-01

Page Start

1,019

Page End

1,030

Print ISSN

0449-2285

Online ISSN

2357-0245

Link

https://ejchem.journals.ekb.eg/article_9151.html

Detail API

https://ejchem.journals.ekb.eg/service?article_code=9151

Order

34

Type

Original Article

Type Code

297

Publication Type

Journal

Publication Title

Egyptian Journal of Chemistry

Publication Link

https://ejchem.journals.ekb.eg/

MainTitle

-

Details

Type

Article

Created At

22 Jan 2023