Beta
ManageSearchChatDiscover
9013

Chemical Characterization of Levan and Optimization of immobilized Bacillus tequilensis levansucrase onto κ-Carrageenan –CMC Gel Beads

Article

Last updated: 01 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Inorganic chemistry

Abstract

B
acillus tequilens was a good levansucrase producer (222.2 U/mL) with levan yield (130 g/L). The levan yield was characterized by FT-IR and the results recorded that the product was mainly fructose. Levansucrase produced by Bacillus tequilens was immobilized by covalent binding on κ-carrageenan and carboxy methyl cellulose gel beads activated by two-step method; the gel beads were soaked in polyethyleneimine followed by glutaraldehyde. Then22 full-factorial central composite experiment designs were employed to optimize the conditions for the maximum enzyme loading efficiency to reach (14.01852 U/of enzyme/g gel beads).The free enzyme showed optimum pH at7 while immobilization process increased the tolerance of enzyme at both alkaline range pH3 and acidic range pH10. The apparent Km after immobilization was 2.85 mg/mL compared to 2.5 mg/mL for free enzyme. Maximum velocity Vmax was 71.4 mg.min−1 for free enzyme while it was 62.4 mg.min−1 for immobilized formula of enzyme. An inhibition of enzyme activity was recognized with all tested metal ion as well as EDETA for either free or immobilized formula of levansucrase.

DOI

10.21608/ejchem.2018.3839.1331

Keywords

Levansucrase, Immobilization, Response surface methodology, Bacillus tequilens

Authors

First Name

Bassem M.

Last Name

Salama

MiddleName

-

Affiliation

Chemistry of Microbial and Natural ProductsDepartment, National Research Centre (NRC); Dokki, Cairo, Egypt.

Email

-

City

-

Orcid

-

First Name

Mohamed E.

Last Name

Hassan

MiddleName

-

Affiliation

Centre of Excellence, Encapsulation Nanobiotechnology Group, National Research Centre, Dokki, Cairo, Egypt.

Email

-

City

-

Orcid

-

First Name

Doaa

Last Name

A. R.

MiddleName

-

Affiliation

Chemistry of Microbial and Natural ProductsDepartment, National Research Centre (NRC); Dokki, Cairo, Egypt.

Email

-

City

-

Orcid

-

First Name

ghada E.A.

Last Name

Awad

MiddleName

-

Affiliation

Chemistry of Microbial and Natural ProductsDepartment, National Research Centre (NRC); Dokki, Cairo, Egypt.

Email

ghadaawadnrc@gmail.com

City

-

Orcid

-

First Name

Naziha M.

Last Name

Hassanein

MiddleName

-

Affiliation

Department of Microbiology, Faculty of Science, Ain Shams Univ., Cairo, Egypt.

Email

-

City

-

Orcid

-

First Name

Wafaa A.

Last Name

Helmy

MiddleName

-

Affiliation

Chemistry of Microbial and Natural ProductsDepartment, National Research Centre (NRC); Dokki, Cairo, Egypt.

Email

-

City

-

Orcid

-

First Name

Mona A.

Last Name

Esawy

MiddleName

-

Affiliation

Chemistry of Microbial and Natural ProductsDepartment, National Research Centre (NRC); Dokki, Cairo, Egypt.

Email

-

City

-

Orcid

-

Volume

61

Article Issue

5

Related Issue

2054

Issue Date

2018-10-01

Receive Date

2018-05-15

Publish Date

2018-10-01

Page Start

857

Page End

866

Print ISSN

0449-2285

Online ISSN

2357-0245

Article File

EJCHEM_Volume 61_Issue 5_Pages 857-866.pdf

PDF . 1.5MB

Link

https://ejchem.journals.ekb.eg/article_9013.html

Detail API

https://ejchem.journals.ekb.eg/service?article_code=9013

Order

10

Type

Original Article

Type Code

297

Publication Type

Journal

Publication Title

Egyptian Journal of Chemistry

Publication Link

https://ejchem.journals.ekb.eg/

MainTitle

Chemical Characterization of Levan and Optimization of immobilized Bacillus tequilensis levansucrase onto κ-Carrageenan –CMC Gel Beads

Details

Type

Article

Created At

22 Jan 2023