A simple reversed phase high performance liquid chromatography method for separation and quantification of tocopherols in edible oils has been developed and validated. In the developed method C18 column was used and a mixture of isopropanol and water was used as mobile phase to achieve baseline separation of all isomers. Tocopherols were separated completely with very good peak resolution in 45 minutes and were detected using diode array detector. No pretreatment of the samples was needed, except dilution in ethanol. The validation measurements showed that the developed method was suitable for quantification of tocopherols in edible oils.