An ecofriendly antimicrobial finishing agent has been prepared from herbal extracts " Chamomile, sage and green tea " which have been applied to cotton fabric in presence and absence of free formaldehyde crosslinking agent (Glyoxal) using pad, dry cure method. Antimicrobial properties of treated fabrics have been improved which make it more important and inevitable finish for garments. Therefore, cotton fabric was treated with each antibacterial extract at various concentrations 1, 2, % bwf using pad-dry –cure method. Treated fabrics have been evaluated for their antimicrobial activity. The Chamomile, sage and green tea treated fabrics exhibited antimicrobial activity against both gram negative and gram positive bacteria and yeasts unicellular fungi. The treated cotton fabrics have shown an excellent antimicrobial activity even after washing 10 cyclic wash. The samples were examined for SEM and physical properties of cotton including, tensile strength and elongation at break Abrasion Resistance, Water & air permeability and Stiffness of the fabric. also the treated fabric show very good results for |UV protection.