The present study concerns the bioremediation process of oil contaminated soil from Suez Oil Processing Company. Nineteen crude oil-degrading bacterial isolates were isolated from this oil-polluted area. Four bacterial species showed the highest growth rate on crude oil hydrocarbons. The bioremediation process was studied by pure and bacterial consortium. The oil contents in the different microcosms reduced up to 80% via bioremediation after 7 days. The Gas Chromatographic analysis of the crude oil remaining in different culture media after one week was determined. The nanoparticle and biosurfactant showed selective bioremediation enhancement for n- and iso- paraffins. The percentage biodegradation was reached into the maximum value in microcosm containing biosurfactant, nanoparticle and bacterial consortium of the best four bacterial strains into 90% after 7 days. The complete degradation of some different members of polyaromatics and the percentage biodegradation of other polyaromatics increased in different microcosms which investigated by HPLC analysis.