2-((2-aminoethyl)amino)-4-aryl-6-indolylnicotinonitriles (1a-c), were grafted into two different cellulosic intermediates, where 6-deoxy tosyl cellulose that was prepared by the reaction of microcrystalline cellulose (MCC) with tosyl chloride in DMA/LiCl. Additionally, the dialdehyde carboxymethyl cellulose (2,3-DACMC) obtained by regioselective periodate oxidation of carboxymethyl cellulose (CMC) were also condensed with the same amine compounds (1a-c) via Schiff's base reaction aiming to obtain new functionalized amino cellulosic derivatives with predictable applications. The products were characterized by FT-IR, 13C-NMR, elemental analysis, SEM, and X-ray diffraction. The antimicrobial activity of the new cellulose amine derivatives and Schiff's bases of (2,3-DACMC) were assayed on four different microorganisms. Among the tested compounds, (3a) and (3b) proved to be promising antimicrobial cellulosic material that could be used as a broad spectrum wound healing gels or manufacturing of packaging materials.