The aim of the present study is to replace chemical surfactant with an alternative substrate containing high level of phospholipids. Phosphotidylcholine (PC) is extracted from soya bean oil waste which is used to obtain non toxic nanoliposome. Nano associated PC/Chol liposome with different specific surfactants were prepared using modified ( REV) method .The results obtained of the dispersed part show that associated liposome with non- ionic and cationic type surfactant show high efficiencies, while with anionic surfactant the efficiency is low. Gas chromatography technique was applied to study the hydrocarbon composition of undispersed part for each type of nanoliposome,the results obtained show that the nanoliposome affected not only on saturated fraction but also on aromatic and iso-paraffin .From TEM and DLS results the particle size of associated liposome showed better distribution than PC/Chol., and the average particle size and zeta potential of all associated liposomes were both reduced for nano-tween liposome and nano-nonylphenol ethoxylate liposome