In this work, amphiphilic ethyl amino-cellulose as oil/water emulsion stabilizer was prepared from bagasse by extraction of cellulose from bleached bagasse pulp. By periodate oxidation of cellulose the corresponding C-2/C-3 dialdehyde cellulose produced, which reacted with ethyl amine to obtain ethyl amino-cellulose (amphiphilic cellulose) through Schiff's base reaction
(reductive amination). The produce ethyl-amino cellulose has a great attention in soybean oil/ water stabilization.The amphiphilic cellulose was characterized by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction, Transmission electron microscopy (TEM), Thermal gravimetric analysis (TGA), and Differential Scanning Calorimetry (DSC). Foureir transform infrared spectroscpy has been proved the incorporation of soybean oil in ethyl amino-cellulose by as their is a new groups appeared in the foureir transform infrared spectra of the prepared emulsion. TEM used to prove the nano-size of the prepared samples. TGA and DSC proved the thermal stability and melting temperature. Sample was successfully applied as stabilizer of soybean oil in water emulsion.