ARAPID and eco-friendly method was devised for fabrication of …….sliver nanoparticles AgNPs at low temperature using honey bee as a reducing agent for silver nitrate and stabilizing agent for AgNPs formed thereof. Formation of AgNPs was evaluated by monitoring UV-vis spectra of the silver colloidal solution, whereas the size of the formed nanoparticles were measured by transmission electron microscopy(TEM). Different reaction parameters pertaining to the synthesis of AgNPs were studied in order to establish optimization. Accordingly, by the most appropriate conditions for preparation of AgNPs colloidal solution having adequate concentration for industrial application, that is, (1080 ppm) were: 10 ml honey bee, 0.17g AgNO3/ 100ml, pH11, temperature 50oC and reaction duration of 60 min. Cotton fabric was treated by the prepared silver nanoparticles colloidal solution using pad-dry curing technique in presence and absence of a binding agent. Thus treated fabrics were evaluated using scanning Electron microscopy (SEM) and bio-assay for antimicrobial activity.