THE SAFETY of healthcare workers has become a serious concern; …..therefore, a need for protection against bacterial penetration and transmission is realized. The purpose of this study is to investigate the functional and antimicrobial properties of cotton: polyester woven fabrics used as garment for healthcare workers uniforms (HCWU). (silver nanoparticles) AgNPs nano finish material is used as antimicrobial agent. The effect of polyester ratio and AgNPs concentration on tensile, water absorbance and antimicrobial properties of these fabrics has been examined. The findings of this study revealed AgNPs can be used effectively as an antimicrobial agent for woven cotton: polyester blended fabrics. The tensile strength, breaking extension, water absorbance of blended woven fabrics are significantly affected by the polyester percentage and the concentration of AgNPs. The produced fabrics were used in garments supplement design for healthcare workers such as doctors, nurse's uniforms.