Background: Thalassemia is inherited disorders of hemoglobin (Hb) synthesis. The clinical severity widely varies, ranging from asymptomatic forms to severe or even fatal form, thalassemia and celiac disease share some clinical features. Growth failure is one of the most common problems in children with thalassemia. Objective: The current study was aimed to determine the frequency of celiac disease in patients with β-thalassemia. Methods: The study was conducted in hematology and oncology unit of Pediatric outpatient Department and clinical pathology department, Zagazig University Hospital during the period from February to December 2018. Subjects & Methods: (86) participants in the study were classified into two groups: Group (1): Including 43 age and sex matched healthy children (21 male and 22 female) as control group. Group (2): Including (43) patients with Beta thalassemia (18 male and 25 female). Results: Our study showed no celiac disease was diagnosed in patients with beta thalassemia involved in the study. Conclusion: serum IgA was higher in patients with beta thalassemia in compared to control groups and no association between β-thalassemia major and celiac disease in the participants involved in the study.