Background: The incidence of diabetes mellitus is increasing worldwide; it has some major effects on the genitourinary system, which makes diabetic patients more liable to urinary tract infection. Despite, all these problems, antibiotics are prescribed empirically which may adversely affect antibiotic resistance so far. Therefore, the aim of this study was to identify the etiologic agents of UTI and their antibiotic susceptibility pattern among diabetic patients attending diabetic clinic of Zagazig University Hospitals. Material and Methods: A cross-sectional study was conducted in a total of 195 diabetic patients who suffered change from June 2017 to June 2018. Demographic and clinical data were collected. Clean catch mid-stream urine samples were collected and processed for identification of uropathogen. Results: E. coli was the commonest isolated uropathogen followed by Klebsiella pneumoniae. All the isolated bacteria were resistant to ceftriaxone but sensitive to ciprofloxacin. Gram-negative isolates demonstrated high level of sensitive to amikacin in 188 (96.4%) patients, imipenem and meropenem in 5 (2.6%) and ceftazidime in 187 (95.9%) patients. Gram-positive bacteria showed sensitive to amoxicillin-clavulanate, linezolid and vancomycin in 7 (3.6%) patients. Multidrug resistance was observed in about 30% of the isolated uropathogens. Conclusion: Pathogens are mostly resistant to antibiotics including ceftriaxzone and ampicillin with few exceptions including nitrofurantoin and sulfamethoxazole-trimethoprim.