Background: Rheumatoid arthritis (RA) is a disease of progressive inflammatory autoimmune nature with articular and systemic effects. Its definite cause is unknown, but environmental and genetic factors are contributory. Aim of the Work: The aim of this study was to estimate the serum level of IL-17 in cases with RA and investigate its relation with disease activity among Egyptian population. Subjects and Methods: This study included 20 RA cases and 20 healthy control subjects. All cases were subjected to full history taking, thorough clinical examination, laboratory investigations including erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), anti-cyclic-citrullinated peptide (anti-CCP) antibodies and rheumatoid factor (RF). Enzyme linked immunosorbent assay was used to measure serum IL-17 level. Disease activity score-28 (DAS-28) was assessed. Results: The mean disease duration of the cases was 5.95±2.52 years (2–10years). DAS-28 ranged between 2.4 and 6.1 with a mean of 3.87±1.32. Serum IL-17 level was higher in the RA group (226.6±215.6 pg/ml) than the control group (48.17±54.9pg/ml) with p-value of 98.2 pg/ml had sensitivity and specificity of 81.2% and 75%, respectively. Conclusion: Serum IL-17 level was significantly increased in cases with RA compared to healthy controls. Its significant correlation with DAS-28 suggested that serum IL-17 level could be an important marker of disease activity in RA.