Introduction: Vitiligo is an acquired skin disease characterized by loss of functional melanocytes from the epidermis. Despitethe several factors studied, the pathogenesis of vitiligo remains unclear. Vitiligo could be associated with low vitamin D levels. The aim of this study was to evaluate serum 25(OH) D levels in vitiligo patients in comparison of normal controls. Methods: After meeting inclusion and exclusion criteria, serum 25 hydroxy vitamin D levels were assayed, in all subjects included in this case control study (21 patients and 21 age and sex matched healthy individuals). Vitiligo disease activity index (VIDA),  affected  body surface area (BSA),site of lesion, age of patients and duration of vitiligo were evaluated  in  relation to vitamin D level. Results: A total of 42 participants were enrolled in our study, 21 patients with vitiligo and 21 who served as controls. The mean serum level of vitamin D were significantly decreased in the patients group as compared with the control group ( 17.3ng ̸ml ± 5.3 vs 25.8 ng/ml ±7.9, P = 0.006). There was non-significant correlation between vitamin D level with age, duration of  vitiligo, and affected  body surface area (P>0.05), but there was significant difference in 25(OH)D levels  between different grades of VIDA. Conclusion: In this study, we found a significant 25(OH) D deficiency in patients with vitiligo, suggesting that vitamin D deficiency may plays a role in the pathogenesis of vitiligo.