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EFFECT OF NESFATIN-1 ON ISCHAEMIA-REPERFUSION INJURY IN ISOLATED HEART OF ALBINO RATS

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Last updated: 01 Jan 2025

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Abstract

Background: Nesfatin-1 is a hypothalamic neuropeptide which is involved in control of food intake and glucose homeostasis. Lower plasma level of Nesfatin-1 in patients with acute myocardial infarction (AMI) was reported.
Objective: This study was designed to explore possible effect of Nesfatin-1 on ischemia-reperfusion injury in isolated heart of adult male albino rats and to explain the possible involved mechanisms, in a trial to clarify Nesfatin-1 expected cardioprotective effect.
Design: This study was carried out on eighteen adult male albino rats which were divided equally (n=6) into 3 groups: Group I (ischemia-reperfusion I/R group); hearts were stabilized then subjected to (I/R) protocol, Group II (Nesfatin-1 pre-conditioning group); Nesfatin-1 was infused for 20 minutes before hearts were subjected to ischemia and Group III (Nesfatin-1 post-conditioning group); Nesfatin-1 was infused for 20 minutes at the beginning of 120 minutes of reperfusion. Cardiac performance indicators as left ventricular pressure (LVP), +max (LV dp/dt), -max (LVdP/dt)+, in addition to heart rate were recorded. Lactate dehydrogenase (LDH), creatine kinase-MB (CK-MB), superoxide dismutase (SOD) and C-reactive protein (CRP) were measured in the collected perfusate and cardiac Malondialdehyde (MDA) was measured. Finally, Nitro blue tetrazolium stain was used to detect the necrotic tissue percentage to the whole left ventricular mass.
Results: In group III (post conditioning group), there was a significant increase of the studied cardiac parameters compared to group I (I/R). Nesfatin-1 significantly increased LVP, +max (dp/dt), -max (dp/dt) and HR in comparison with I/R group. This was associated with a significant decrease in LDH and CK-MB levels, a significant increase in SOD level and a significant decrease in MDA and CRP levels. Moreover, Nesfatin-1 caused a significant decrease in percentage of necrotic tissue to the whole left ventricular mass. Regarding group II, no significant changes were detected in all parameters except for significant increase in SOD level and significant decrease in CRP level.
Conclusion: Nesfatin-1 protects against ischemia/reperfusion injury in vitro through its antioxidant and anti-inflammatory properties by limiting the infarction area, only if given as a post conditioning factor after I/R. Those results open the way to include Nesfatin-1 among the strategies for management of cardiac infarction during reperfusion.

DOI

10.21608/zumj.2016.4671

Keywords

Nesfatin-1, cardiac ischemia-reperfusion, Oxidative Stress, Inflammation

Authors

First Name

Mohamed

Last Name

Tawfik

MiddleName

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Affiliation

Physiology Department, Faculty of Medicine, Zagazig University

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Orcid

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First Name

Dalia

Last Name

Abd Al-Aleem

MiddleName

-

Affiliation

Physiology Department, Faculty of Medicine, Zagazig University

Email

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City

-

Orcid

-

First Name

Reham

Last Name

Ibrahim

MiddleName

-

Affiliation

Physiology Department, Faculty of Medicine, Zagazig University

Email

-

City

-

Orcid

-

First Name

Rehab

Last Name

Abaza

MiddleName

-

Affiliation

Physiology Department, Faculty of Medicine, Zagazig University

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-

City

-

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-

Volume

22

Article Issue

6

Related Issue

772

Issue Date

2016-11-01

Receive Date

2017-12-18

Publish Date

2016-11-01

Page Start

1

Page End

11

Print ISSN

1110-1431

Online ISSN

2357-0717

Link

https://zumj.journals.ekb.eg/article_4671.html

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https://zumj.journals.ekb.eg/service?article_code=4671

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Original Article

Type Code

273

Publication Type

Journal

Publication Title

Zagazig University Medical Journal

Publication Link

https://zumj.journals.ekb.eg/

MainTitle

EFFECT OF NESFATIN-1 ON ISCHAEMIA-REPERFUSION INJURY IN ISOLATED HEART OF ALBINO RATS

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Article

Created At

22 Jan 2023